Interference studies in Neurospora crassa and N. sitophila

Heterokaryosis in Neurospora sitophila.

ETEROKARYOSIS has been extensively used as a major tool in formal genetic analysis of both Mendelian and non-Mendelian genes and in establishing gene-enzyme relationships. So far it is the only way to perform genetic anslysis of somatic cells in tissue culture (EPHRUSSI 1965; MINTZ and BAKER 1967). These aspects of heterokaryosis have been reviewed elsewhere ( JINKS 1965; FINCHAM 1966; DAVIS 19...

Quelling in Neurospora crassa.

Heterocaryosis in Neurospora Crassa.

T IS possible by means of X-ray or ultraviolet treatment to produce mutant I strains of Neurospora crassa that are deficient in their abilities to synthesize specific vitamins, amino acids, or other essential substances (BEADLE and TATUM 1941; TATUM and BEADLE 1g4zb). It occurred to us that it might be possible to determine whether the mutant genes concerned in such haploid strains are recessiv...

Biochemical and genetic studies on galactosamine metabolism in Neurospora crassa.

In Neurospora, galactosamine can be released from the cell wall and from an alcohol-soluble compound by acid hydrolysis. All of the detectable alcohol-soluble galactosamine was present as uridine diphospho-2-acetamido-2-deoxy-D-galactose (UDPGalNAc). The results of pulse-labeling studies and enzymatic assays indicated that UDPGalNAc was synthesized via the epimerization of uridine diphospho-2-a...

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...